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Human eIF4A2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-31554

Novus Biologicals, part of Bio-Techne
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NBP3-31554

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.059 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human eIF4A2 ELISA Kit (Colorimetric)

Human eIF4A2 ELISA Kit (Colorimetric)

ELISA: Human eIF4A2 ELISA Kit (Colorimetric) [NBP3-31554] -

ELISA: Human eIF4A2 ELISA Kit (Colorimetric) [NBP3-31554] - Standard Curve Reference

Kit Contents for Human eIF4A2 ELISA Kit (Colorimetric)

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: eIF4A2

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Alternate Names

ATP-dependent RNA helicase eIF4A-2, BM-010, DDX2Beukaryotic translation initiation factor 4A, isoform 2, EC 3.6.1, EC 3.6.4.13, EIF4A, eIF-4A-II, eIF4A-II, EIF4F, eukaryotic initiation factor 4A-II, eukaryotic translation initiation factor 4A, eukaryotic translation initiation factor 4A2

Gene Symbol

EIF4A2

Additional eIF4A2 Products

Product Documents for Human eIF4A2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human eIF4A2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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