Estradiol Parameter Assay Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # KGE014
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
12.1 pg/mL
Assay Range
12.3-3000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Estradiol Parameter Assay Kit
The Parameter Estradiol Immunoassay is a 3.5 hour competitive enzyme
immunoassay designed to measure Estradiol in cell culture supernates, serum, and plasma.
Product Specifications
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Reactivity
Multi-Species
Specificity
Measures Estradiol levels in various samples. This assay cross-reacts 0.07% with 17 alpha-ethynylestradiol, 0.03% with 17 beta-estradiol 3-Benzoate, 0.86% with Estriol, 0.26% with Estrone and 0.06% with Progesterone.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 153 | 744 | 1419 | 164 | 765 | 1533 |
| Standard Deviation | 13.6 | 26.8 | 76.6 | 9.54 | 61.5 | 116 |
| CV% | 8.9 | 3.6 | 5.4 | 5.8 | 8.0 | 7.6 |
Recovery for Estradiol Parameter Assay Kit
The recovery of Estradiol spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 94 | 81-102 |
| EDTA Plasma (n=4) | 103 | 90-113 |
| Heparin Plasma (n=4) | 102 | 88-117 |
| Serum (n=4) | 96 | 87-108 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Estradiol were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. <br>
Scientific Data Images for Estradiol Parameter Assay Kit
Multi-species Estradiol ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Estradiol
Alternate Names
Estrogen
Additional Estradiol Products
Product Documents for Estradiol Parameter Assay Kit
Product Specific Notices for Estradiol Parameter Assay Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.