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Human FGF-12 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-31615

Novus Biologicals, part of Bio-Techne
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NBP3-31615

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids (100 uL)

Sensitivity

8.1 pg/mL (example only; lot dependent)

Assay Range

15.625 - 1000 pg/mL (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human FGF-12 ELISA Kit (Colorimetric)

Human FGF-12 ELISA Kit (Colorimetric)

ELISA: Human FGF-12 ELISA Kit (Colorimetric) [NBP3-31615] -

ELISA: Human FGF-12 ELISA Kit (Colorimetric) [NBP3-31615] - Standard Curve Reference

Kit Contents for Human FGF-12 ELISA Kit (Colorimetric)

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: FGF-12

FGF-12 is a member of the FGF superfamily of heparin-binding mitogenic molecules characterized by the presence of a core, 120 amino acid (aa) beta-trefoil structure. FGF-11, -12, -13, -14, originally termed FGF homologous factors (FHF) -3, -1, -2, and -4, respectively, form a subgroup within the FGF family. Human FGF-12/FHF-1 is synthesized as a 243 aa protein. It lacks a typical signal sequence and is considered cytoplasmic. It also possess an N-terminal bipartite nuclear localization signal at aa 11-18 and 28-38.

Long Name

Fibroblast Growth Factor 12

Alternate Names

FGF12, FHF1

Gene Symbol

FGF12

Additional FGF-12 Products

Product Documents for Human FGF-12 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human FGF-12 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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