Human GAD2/GAD65 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18768

Novus Biologicals, part of Bio-Techne
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NBP3-18768
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

46.88 pg/mL

Assay Range

78.13-5000 pg/mL

View all GAD2/GAD65 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human GAD2/GAD65 in samples. No significant cross-reactivity or interference between Human GAD2/GAD65 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.40

Inter-Assay Precision (Precision between assays) %CV < 5.48

Recovery for Human GAD2/GAD65 ELISA Kit (Colorimetric)

Recovery

87-107%

Linearity

Human GAD2/GAD65 ELISA Kit (Colorimetric)

Scientific Data Images for Human GAD2/GAD65 ELISA Kit (Colorimetric)

Human GAD2/GAD65 ELISA Kit (Colorimetric)

ELISA: Human GAD2/GAD65 ELISA Kit (Colorimetric) [NBP3-18768] -

ELISA: Human GAD2/GAD65 ELISA Kit (Colorimetric) [NBP3-18768] - Standard Curve Reference

Kit Contents for Human GAD2/GAD65 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GAD2/GAD65

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Long Name

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)

Alternate Names

GAD65

Gene Symbol

GAD2

Additional GAD2/GAD65 Products

Product Documents for Human GAD2/GAD65 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human GAD2/GAD65 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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