Human Galectin-3 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91275

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA), buffered solution (50 uL)

Sensitivity

0.12 ng/mL

Assay Range

0.39 - 25.0 ng/mL

View all Galectin-3 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The assay detects both natural and recombinant Galectin-3. The crossreactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a Galectin-3 positive sample. No cross-reactivity or interference was detected.

Description

The human Galectin-3 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Galectin-3

Precision

Intra-Assay Precision (Precision within an assay) CV% < 7.50%

Inter-Assay Precision (Precision between assays) CV% < 5.40%

Recovery for Human Galectin-3 ELISA Kit (Colorimetric)

Recovery

Please See Protocol for Specific Spike Recovery Details

Scientific Data Images for Human Galectin-3 ELISA Kit (Colorimetric)

ELISA: Human Galectin-3 ELISA Kit (Colorimetric) [NBP1-91275]

ELISA: Human Galectin-3 ELISA Kit (Colorimetric) [NBP1-91275]

ELISA: Galectin-3 ELISA Kit [NBP1-91275] - Standard curve

Kit Contents for Human Galectin-3 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Galectin-3
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • HRP-Conjugate anti-human Galectin-3 monoclonal antibody
  • Human Galectin-3 Standard lyophilized, 60 ng/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Formulation

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Galectin-3

The human Galectin-3 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Galectin-3. Galectin-3 is a 26kDa -galactoside-binding protein belonging to the Galectin family, which consists of more than ten members. Galectin-3 is composed of a carboxyl-terminal carbohydrate recognition domain (CRD) and amino-terminal tandem repeats. Galectin-3 normally distributes in epithelia of many organs and various inflammatory cells, including macrophages as well as dendritic cells and Kupfer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation and cell differentiation and through transactivation by viral proteins. Galectin-3 has been shown to function through both intracellular and extracellular actions. It is a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing and has been found to control cell cycle and prevent T-cell apoptosis through interaction with the Bcl-2 family members. On the other hand, this protein, which is secreted from monocytes/macrophages and epithelial cells has been demonstrated to function as an extracellular molecule in activating various types of cells such as monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions and acts as a novel chemoattractant for monocytes and macrophages.

Alternate Names

AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2

Gene Symbol

LGALS3

Additional Galectin-3 Products

Product Documents for Human Galectin-3 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Galectin-3 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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