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Human Gastric Lipase ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00409

Novus Biologicals, part of Bio-Techne
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NBP3-00409

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Gastric Lipase in samples. No significant cross-reactivity or interference between Human Gastric Lipase and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.96%

Inter-Assay Precision (Precision between assays) 5.42%

Recovery for Human Gastric Lipase ELISA Kit (Colorimetric)

Recovery

86 - 111%

Linearity

Human Gastric Lipase ELISA Kit (Colorimetric)

Scientific Data Images for Human Gastric Lipase ELISA Kit (Colorimetric)

Human Gastric Lipase ELISA Kit (Colorimetric)

ELISA: Human Gastric Lipase ELISA Kit (Colorimetric) [NBP3-00409] -

ELISA: Human Gastric Lipase ELISA Kit (Colorimetric) [NBP3-00409] - Standard Curve Reference

Kit Contents for Human Gastric Lipase ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Gastric Lipase

Gastric Lipase encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism.

Long Name

Gastric triacylglycerol lipase

Alternate Names

GL, LIPF

Gene Symbol

LIPF

Additional Gastric Lipase Products

Product Documents for Human Gastric Lipase ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Gastric Lipase ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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