Human Gastrin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-31751

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids (50 uL)

Sensitivity

4.36 pg/mL (example only; lot dependent)

Assay Range

15.625 - 1000 pg/mL (example only; lot dependent)

View all Gastrin I ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Gastrin ELISA Kit (Colorimetric)

Human Gastrin ELISA Kit (Colorimetric)

ELISA: Human Gastrin ELISA Kit (Colorimetric) [NBP3-31751] -

ELISA: Human Gastrin ELISA Kit (Colorimetric) [NBP3-31751] - Standard Curve Reference

Kit Contents for Human Gastrin ELISA Kit (Colorimetric)

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Gastrin I

Gastrin, which is normally formed by mucosal cells in the gastric antrum and by the D cells of the pancreatic islets, is a hormone whose main function is to stimulate secretion of HCl by the gastric mucosa. HCl, in turn, inhibits gastrin formation. Gastrin also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. Gastrin is regulated by epidermal growth factor in both mice and humans. Gastrin is excreted in excess by pancreatic tumors in the Zollinger- Ellison syndrome. Gastrin maps to human chromosome 17q-21. Gastrin- Releasing Peptide (GRP) stimulates the release of gastrin as well as other gastrointestinal hormones, in addition to acting as an autocrine growth factor for certain cell types. High levels of GRP are found in the human lung just after birth and levels decrease thereafter in parallel with the observed disease in a number of pulmonary neuroendocrine cells. GRP is known to promote lung tumorigenesis in model systems and, interestingly, is induced by retinoic acid. GRP is involved in several functions with the hypothalamus, and is thought to play a role in regulating pituitary hormone secretion. GRP maps to human chromosome 18q21.

Alternate Names

G17, GAST, Gastrin-17

Gene Symbol

GAST

Additional Gastrin I Products

Product Documents for Human Gastrin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Gastrin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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