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Human GLDC - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-31811

Novus Biologicals, part of Bio-Techne
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NBP3-31811

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.132 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human GLDC - Ready-To-Use ELISA Kit (Colorimetric)

Human GLDC - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human GLDC  - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31811] -

ELISA: Human GLDC - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31811] - Standard Curve Reference

Kit Contents for Human GLDC - Ready-To-Use ELISA Kit (Colorimetric)

  • Detection Solution A
  • Detection Solution B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GLDC

The enzyme system for cleavage of glycine (glycine cleavage system; GCS; EC 2.1.2.10), which is confined to themitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase),H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (alipoamide dehydrogenase). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes;see MIM 238310, MIM 238330, and MIM 238331.(supplied by OMIM)

Alternate Names

EC 1.4.4.2, GCE, GCSPMGC138198, Glycine cleavage system P protein, glycine cleavage system protein P, Glycine decarboxylase, glycine decarboxylase P-protein, glycine dehydrogenase (decarboxylating), glycine dehydrogenase (decarboxylating; glycine decarboxylase, glycine cleavagesystem protein P), glycine dehydrogenase [decarboxylating], mitochondrial, HYGN1, MGC138200, NKH

Gene Symbol

GLDC

Additional GLDC Products

Product Documents for Human GLDC - Ready-To-Use ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human GLDC - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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