Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67990

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.09 mIU/mL

Assay Range

0.16-10 ng/mL

View all Glutamate Dehydrogenase 1/GLUD1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human GDH/GLDH in samples. No significant cross-reactivity or interference between Human GDH/GLDH and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10.19%

Inter-Assay Precision (Precision between assays) CV% < 9.65%

Recovery for Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence)

Recovery

88-107%

Linearity

Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence)

Scientific Data Images for Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence)

Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence)

ELISA: Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence) [NBP2-67990] -

ELISA: Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence) [NBP2-67990] - Standard Curve Reference

Kit Contents for Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Glutamate Dehydrogenase 1/GLUD1

One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1; EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.

Alternate Names

GDH, GDH1

Gene Symbol

GLUD1

Additional Glutamate Dehydrogenase 1/GLUD1 Products

Product Documents for Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Glutamate Dehydrogenase ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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