Mouse GM-CSF ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92674

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

2.0 pg/mL

Assay Range

3.9 - 250 pg/mL

View all GM-CSF ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse GM-CSF positive serum. There was no crossreactivity detected.

Description

The mouse GM-CSF ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse GM-CSF.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.0%

Inter-Assay Precision (Precision between assays) CV% < 10%

Recovery for Mouse GM-CSF ELISA Kit (Colorimetric)

Recovery

The overall mean recovery was 85%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Mouse GM-CSF ELISA Kit (Colorimetric)

ELISA: Mouse GM-CSF ELISA Kit (Colorimetric) [NBP1-92674]

ELISA: Mouse GM-CSF ELISA Kit (Colorimetric) [NBP1-92674]

ELISA: Mouse GM-CSF ELISA Kit (Colorimetric) [NBP1-92674] - Standard curve using mouse GM-CSF ELISA Kit.

Kit Contents for Mouse GM-CSF ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse GM-CSF
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-mouse GM-CSF monoclonal antibody
  • Mouse GM-CSF Standard lyophilized, 500 pg/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GM-CSF

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that has been characterized as a molecule capable of stimulating proliferation and differentiation of granulocyte/macrophage progenitor cells as well as modulating the functional activity of mature granulocytes and macrophages. In combination with erythropoietin, it supports the development of erythroid progenitors in vitro. The biological activities of GM-CSF substantially overlap with the effects of Interleukin 3. The reason for the redundancy of these pleiotropic cytokines may at least in part be explained by their competition for binding to common receptors. GM-CSF is produced by a number of different cell types such as activated T-cells, B-cells, macrophages, mast cells, endothelial cells, fibroblasts, synoviocytes and various tumor cells in response to various stimuli. Mouse GM-CSF as well as its receptors have been cloned. The encoded glycoprotein consists of 141 amino acid residues and has a molecular weight of about 23 kDa. The murine protein binds to a high affinity receptor complex consisting of two membrane glycoproteins, the GM-CSF receptor alpha subunit with notable homology to the human receptor and the beta subunit which also forms part of the receptors for IL-3 and IL-5. Elevated levels of GM-CSF are found in mice after endotoxin injection.

Long Name

Granulocyte Macrophage Growth Factor

Alternate Names

CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim

Gene Symbol

CSF2

Additional GM-CSF Products

Product Documents for Mouse GM-CSF ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse GM-CSF ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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