Human GRK4 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38940

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.062 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

View all GRK4 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human GRK4 ELISA Kit (Colorimetric)

Human GRK4 ELISA Kit (Colorimetric)

ELISA: Human GRK4 ELISA Kit (Colorimetric) [NBP3-38940]

ELISA: Human GRK4 ELISA Kit (Colorimetric) [NBP3-38940] - Standard Curve Reference

Kit Contents for Human GRK4 ELISA Kit (Colorimetric)

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GRK4

A guanine nucleotide-binding protein (G Protein)-coupled receptor kinase subfamily, of the Ser/Thr protein kinase family, is encoded by the gene GRK4. There are a total of four isoforms of this gene: GRK4-alpha is 578 amino acids long at 66 kDA; GRK4-beta is 546 amino acids long at 63 kDA; GRK4-delta is 500 amino acids long at 57 kDA; and isoform 4, GRK4-gamma is 532 amino acids long at 61 kDA. GRK4-alpha is inhibited by caulmodulin and can phosphorylate rhodopsin, however the three other forms of GRK4 do not interact with calmodulin. GRK4 has been investigated in hypertension, breast cancer, aortic coarctation, and atherosclerosis. It participates in endocytosis and the chemokine signaling pathway through interactions with the CALM1, CALM2, CALM3, FSHR, and IKBKG genes.

Alternate Names

EC 2.7.11, EC 2.7.11.16, G protein-coupled receptor kinase 2-like (Drosophila), G protein-coupled receptor kinase 4, G protein-coupled receptor kinase GRK4, GPRK2LIT11, GPRK4GRK4a, G-protein coupled receptor kinase 4, ITI1

Gene Symbol

GRK4

Additional GRK4 Products

Product Documents for Human GRK4 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human GRK4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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