Human GRP78/HSPA5 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82201

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

0.38 ng/mL

Assay Range

0.63 - 40 ng/mL

View all GRP78/HSPA5 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human GRP78/HSPA5 in samples. No significant cross-reactivity or interference between Human GRP78/HSPA5 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6%

Inter-Assay Precision (Precision between assays) CV% < 5%

Recovery for Human GRP78/HSPA5 ELISA Kit (Colorimetric)

Recovery

average = 99% for serum; average = 96% for EDTA plasma; average = 95% for cell culture media

Linearity

Human GRP78/HSPA5 ELISA Kit (Colorimetric)

Scientific Data Images for Human GRP78/HSPA5 ELISA Kit (Colorimetric)

Human GRP78/HSPA5 ELISA Kit (Colorimetric)

ELISA: Human GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-82201] -

ELISA: Human GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-82201] - Standard Curve Reference

Kit Contents for Human GRP78/HSPA5 ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GRP78/HSPA5

Within the ER, misfolded proteins are detected by Bip (GPR78). In the presence of misfolded proteins, GPR78 disscociates from PERK, allowing it to homodimerize and autophosphoyate. In its dimerized phosphorylated form, PERK phosphorylates eIF2. The phosphorylation of eIF2 blocks the majority of translation to prevent the continued accumulation of protein in the ER. GRP78 also binds to IRE1 and ATF6 under normal ER conditions, but dissociates upon accumulation of misfolded proteins. Unbound ATF6 is translocated to the gogli where it is converted into a cleaved, active form. The cleaved form of ATF6 is then able to up regulate transcription of UPR genes including XBP1. Activated IRE1 acts as an endoribonuclease to an intron from XBP1 transcripts. The XBP1 splice variant codes for an active transcription factor which activates transcription of P58IPK. P58IPK is a HSP40 protein which binds to and inhibits PERK. Thus, if the accumulated misfolded proteins have been removed, P58IPK acts to shut down the UPR by removing the block to translation.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

BIP, HSP70-5, HSPA5

Gene Symbol

HSPA5

Additional GRP78/HSPA5 Products

Product Documents for Human GRP78/HSPA5 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human GRP78/HSPA5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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