Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82130

Novus Biologicals, part of Bio-Techne
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NBP2-82130
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell supernatant (100 uL)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL

View all Hepcidin ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Hepcidin Antimicrobial Peptide in samples. No significant cross-reactivity or interference between Rat Hepcidin Antimicrobial Peptide and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5%

Inter-Assay Precision (Precision between assays) CV% < 6%

Recovery for Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric)

Recovery

average = 95% for serum; average = 97% for EDTA plasma; average = 97% for cell culture media

Linearity

Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric)

Scientific Data Images for Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric)

Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric)

ELISA: Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric) [NBP2-82130] -

ELISA: Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric) [NBP2-82130] - Standard Curve Reference

Kit Contents for Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Hepcidin

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure.

Long Name

Hepcidin Antimicrobial Peptide

Alternate Names

HAMP, HEPC, HFE2B, LEAP1, PLTR

Gene Symbol

HAMP

Additional Hepcidin Products

Product Documents for Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Hepcidin Antimicrobial Peptide ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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