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Human Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76594

Novus Biologicals, part of Bio-Techne
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NBP2-76594

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.188ng/mL

Assay Range

0.313 - 20ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human HSL in samples. No significant cross-reactivity or interference between Human HSL and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.71

Inter-Assay Precision (Precision between assays) CV% < 4.6

Recovery for Human Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric)

Recovery

84-108%

Linearity

Human Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric)

Scientific Data Images for Human Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric)

Human Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric)

ELISA: Human Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric) [NBP2-76594] -

ELISA: Human Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric) [NBP2-76594] - Standard Curve Reference

Kit Contents for Human Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Hormone-sensitive Lipase/HSL

Hormone-sensitive lipase (HSL) is an intracellular neutral lipase found in a variety of tissues, but primarily in adipose and steroidogenic tissues. It hydrolyzes triglycerides into free fatty acids and cholesteryl esters into free cholesterol for steroid hormone synthesis.

HSL is the major enzyme responsible for the mobilization of free fatty acids from adopise tissue, and HSL antibodies are useful tools for lipid and metabolism research.

Alternate Names

Hormonesensitive Lipase, HSL, LHS, LIPE

Gene Symbol

LIPE

Additional Hormone-sensitive Lipase/HSL Products

Product Documents for Human Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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