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Human HPRG ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80315

Novus Biologicals, part of Bio-Techne
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NBP2-80315

Key Product Details

Sample Type & Volume Required Per Well

Serum (100 ul)

Sensitivity

9.70 pg/ml

Assay Range

18.75-1200 pg/ml (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Conjugate

HRP

Reactivity

Human

Specificity

Recognizes both recombinant and natural Human HPRG.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.2

Inter-Assay Precision (Precision between assays) CV% < 8.9 %

Recovery for Human HPRG ELISA Kit (Colorimetric)

Recovery

93 -94%

Scientific Data Images for Human HPRG ELISA Kit (Colorimetric)

Human HPRG ELISA Kit (Colorimetric) [NBP2-80315] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Kit Contents for Human HPRG ELISA Kit (Colorimetric)

  • 1. 96 well microplate coated with Capture Antibody
  • 2. Detection Antibody conjugated to HRP
  • 3. Standards
  • 4. Wash Buffer Concentrate
  • 5. Dilution Buffer Concentrate
  • 6. Color Reagent A
  • 7. Color Reagent B
  • 8. Stop Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage is content dependent.

Background: HPRG

HRG, also known as Histidine-rich glycoprotein, is a 525 amino acid protein that is approx. 60 kDa, functions as plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions; acts as an adapter protein; mediates clearance of necrotic cells; inhibits the formation of insoluble immune complexes; ties plasminogen to the cell surface; binds T-cells and alters the cell morphology; modulates angiogenesis; inhibits endothelial cell motility; plays a role in the regulation of tumor angiogenesis and tumor immune surveillance; and normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages. Studies on this protein have shown a relationship with thrombophilia, thrombophilia due to elevated hrg, thrombophilia due to hrg deficiency, protein c deficiency, myocardial infarction, acute myocardial infarction, corneal neovascularization, thrombosis, rheumatoid arthritis, hyperhomocysteinemia, arthritis, retinal vein occlusion, graft versus host disease, nephrotic syndrome, diabetes mellitus, cd3, twinning, retinitis, and hepatitis b. This protein has been shown to have interactions with C1QB, FGA, C1QA, FCGR1A, PLG, and THBS1 in the hemostasis, platelet degranulation, dissolution of fibrin clot, platelet activation, signaling and aggregation, and response to elevated platelet cytosolic Ca2+ pathways.

Long Name

Histidine-rich Glycoprotein Precusor

Alternate Names

HRG

Gene Symbol

HRG

Additional HPRG Products

Product Documents for Human HPRG ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human HPRG ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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