Human HR6A/UBE2A ELISA Kit (Colorimetric)

Name: Human HR6A/UBE2A ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-38780

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38780

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids (100 uL)

Sensitivity

0.081 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human HR6A/UBE2A ELISA Kit (Colorimetric)

ELISA: Human HR6A/UBE2A ELISA Kit (Colorimetric) [NBP3-38780]

ELISA: Human HR6A/UBE2A ELISA Kit (Colorimetric) [NBP3-38780] - Standard Curve Reference

Kit Contents for Human HR6A/UBE2A ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HR6A/UBE2A

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development. NCOA6 is over-expressed in several breast cancer cell lines.

Long Name

Ubiquitin-conjugating Enzyme E2A

Alternate Names

RAD6A, UBE2A

Gene Symbol

UBE2A

Additional HR6A/UBE2A Products

Product Documents for Human HR6A/UBE2A ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human HR6A/UBE2A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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