Rat HSF2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76593

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.094ng/mL

Assay Range

0.156 - 10ng/mL

View all HSF2 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat HSF2 in samples. No significant cross-reactivity or interference between Rat HSF2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.29

Inter-Assay Precision (Precision between assays) CV% < 4.49

Recovery for Rat HSF2 ELISA Kit (Colorimetric)

Recovery

90-105%

Linearity

Rat HSF2 ELISA Kit (Colorimetric)

Scientific Data Images for Rat HSF2 ELISA Kit (Colorimetric)

Rat HSF2 ELISA Kit (Colorimetric)

ELISA: Rat HSF2 ELISA Kit (Colorimetric) [NBP2-76593] -

ELISA: Rat HSF2 ELISA Kit (Colorimetric) [NBP2-76593] - Standard Curve Reference

Kit Contents for Rat HSF2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HSF2

Heat shock factor 1 (HSF1) is a heat shock transcription factor that activates the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. Up-regulation of expression of heat shock proteins in response to stress occurs at the level of transcription through a heat shock element and a HSF transcription factor. Amino acid sequences for most HSFs are highly conserved. A DNA binding domain is at the N-terminus, hydrophobic repeats (essential to the formation of active trimers) are adjacent to this binding domain, and another short hydrophobic repeat (necessary for suppression of trimerization) occurs toward the C-terminus. HSF2 exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form. Various experiments have suggested that HSF2 may play roles in differentiation and development.

Long Name

Heat Shock Transcription Factor 2

Alternate Names

HSTF2

Gene Symbol

HSF2

Additional HSF2 Products

Product Documents for Rat HSF2 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat HSF2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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