Human HSP40/DNAJB1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76440

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all HSP40/DNAJB1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human HSP-40 in samples. No significant cross-reactivity or interference between Human HSP-40 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.19%

Inter-Assay Precision (Precision between assays) CV% < 5.75%

Recovery for Human HSP40/DNAJB1 ELISA Kit (Colorimetric)

Recovery

89-111%

Linearity

Human HSP40/DNAJB1 ELISA Kit (Colorimetric)

Scientific Data Images for Human HSP40/DNAJB1 ELISA Kit (Colorimetric)

Human HSP40/DNAJB1 ELISA Kit (Colorimetric)

ELISA: Human HSP40/DNAJB1 ELISA Kit (Colorimetric) [NBP2-76440] -

ELISA: Human HSP40/DNAJB1 ELISA Kit (Colorimetric) [NBP2-76440] - Standard Curve Reference

Kit Contents for Human HSP40/DNAJB1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: HSP40/DNAJB1

Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins.

Long Name

Heat Shock 40 kDa Protein

Alternate Names

DNAJB1, Hdj1, HSPF1

Gene Symbol

DNAJB1

Additional HSP40/DNAJB1 Products

Product Documents for Human HSP40/DNAJB1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human HSP40/DNAJB1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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