Human ACE Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DACE00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), Heparin Plasma (10 µL), Saliva (25 µL)
Sensitivity
0.051 ng/mL
Assay Range
0.78-50 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva)
Product Summary for Human ACE Quantikine ELISA Kit
The Quantikine Human ACE Immunoassay is a 4.5 hour solid phase ELISA designed to measure ACE in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed ectodomain of recombinant human sACE, and antibodies raised against the recombinant factor. Natural human ACE showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human ACE.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human ACE. In addition to soluble ACE, this kit recognizes membrane-bound forms from lysates of cultured cells and supernates.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 3.60 | 10.7 | 22.1 | 3.44 | 10.3 | 21.8 |
| Standard Deviation | 0.08 | 0.30 | 0.95 | 0.21 | 0.40 | 0.87 |
| CV% | 2.2 | 2.8 | 4.3 | 6.1 | 3.9 | 4.0 |
Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 3.57 | 10.9 | 22.5 | 3.77 | 11.7 | 24.4 |
| Standard Deviation | 0.12 | 0.43 | 0.90 | 0.29 | 0.60 | 1.2 |
| CV% | 3.4 | 3.9 | 4.0 | 7.7 | 5.1 | 4.9 |
Recovery for Human ACE Quantikine ELISA Kit
The recovery of ACE spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 98 | 89-106 |
| Heparin Plasma (n=4) | 100 | 94-106 |
| Serum (n=4) | 99 | 91-106 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of ACE were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human ACE Quantikine ELISA Kit
Human ACE/CD143 ELISA Cell Culture Supernate/Saliva Standard Curve
Human ACE/CD143 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ACE/CD143
ACE and ACE-2, two cell surface proteases, are important regulators of the renin-angiotensin system (RAS), which plays a key role in maintaining blood pressure homeostasis and fluid salt balance in mammals. Because of its location and specificity, ACE plays additional roles in immunity, reproduction and neuropeptide regulation. ACE exists in two isoforms. Somatic ACE comprises two highly similar protease domains whereas germinal ACE comprises a single protease domain. ACE-2 consists of a single protease domain and is an essential regulator of heart and lung function. It is also a cellular receptor for the Spike protein of SARS cornonavirus that causes severe acute respiratory syndrome.
Long Name
Angiotensin I Converting Enzyme
Alternate Names
CD143, DCP1, Kininase II
Gene Symbol
ACE
Additional ACE/CD143 Products
Product Documents for Human ACE Quantikine ELISA Kit
Product Specific Notices for Human ACE Quantikine ELISA Kit
For research use only
Loading...
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.