Human AgRP Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DAGR00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (25 µL)
Sensitivity
2.68 pg/mL
Assay Range
7.81-500 pg/mL (Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human AgRP Quantikine ELISA Kit
The Quantikine Human AgRP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human AgRP in cell culture supernates, plasma, and urine. It contains Sf 21-expressed recombinant human AgRP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human AgRP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human AgRP.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human AgRP
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 54.3 | 143 | 257 | 43.7 | 126 | 238 |
| Standard Deviation | 2.64 | 3.88 | 5.69 | 3.91 | 8.24 | 16.3 |
| CV% | 4.9 | 2.7 | 2.2 | 8.9 | 6.5 | 6.8 |
EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 56.6 | 144 | 262 | 45.1 | 130 | 246 |
| Standard Deviation | 3.14 | 4.86 | 7.82 | 4.66 | 10.0 | 18.1 |
| CV% | 5.5 | 3.4 | 3.0 | 10.3 | 7.7 | 7.4 |
Recovery for Human AgRP Quantikine ELISA Kit
The recovery of AgRP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 106 | 95-115 |
| EDTA Plasma (n=4) | 96 | 89-106 |
| Heparin Plasma (n=4) | 92 | 84-103 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of AgRP were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human AgRP Quantikine ELISA Kit
Human AgRP/ART ELISA Calibrator Diluent RD5-23 Standard Curve
Human AgRP/ART ELISA Calibrator Diluent RD6-10 Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: AgRP/ART
Agouti-related Protein (AgRP), also known as the protein product of the Agouti-related Transcript (ART), is a neuropeptide and a functional antagonist of alpha-MSH on Melanocortin (MC)-3 and MC-4 receptors, both of which are implicated in obesity. AgRP shares sequence and structural similarity with the Agouti protein and has similar expression patterns in both mouse and human.
Long Name
Agouti-related Protein
Alternate Names
ART
Gene Symbol
AGRP
Additional AgRP/ART Products
Product Documents for Human AgRP Quantikine ELISA Kit
Product Specific Notices for Human AgRP Quantikine ELISA Kit
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.