Human alpha 1-Acid Glycoprotein Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DAGP00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)
Sensitivity
0.538 ng/mL
Assay Range
3.12-200 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human alpha 1-Acid Glycoprotein Quantikine ELISA Kit
The Quantikine Human AGP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human AGP in cell culture supernates, serum, plasma, and urine.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural human AGP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 16.5 | 45.8 | 105 | 16.7 | 45.2 | 105 |
| Standard Deviation | 0.87 | 1.96 | 7.75 | 1.11 | 2.96 | 8.60 |
| CV% | 5.3 | 4.3 | 7.4 | 6.7 | 6.6 | 8.2 |
Recovery for Human alpha 1-Acid Glycoprotein Quantikine ELISA Kit
The recovery of AGP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 98 | 85-111 |
| Urine (n=4) | 96 | 86-111 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of AGP were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human alpha 1-Acid Glycoprotein Quantikine ELISA Kit
Human alpha 1-Acid Glycoprotein ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: alpha 1-Acid Glycoprotein
Alternate Names
AGP, AGP1, AGPA, alpha 1Acid Glycoprotein, ORM1, Orosomucoid
Gene Symbol
ORM1
Additional alpha 1-Acid Glycoprotein Products
Product Documents for Human alpha 1-Acid Glycoprotein Quantikine ELISA Kit
Product Specific Notices for Human alpha 1-Acid Glycoprotein Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.