Human alpha-Fetoprotein Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DAFP00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
0.046 ng/mL
Assay Range
0.312-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human alpha-Fetoprotein Quantikine ELISA Kit
The Quantikine Human AFP immunoassay is a 4.5 hour solid phase ELISA designed to measure human AFP in cell culture supernates, serum, and plasma.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural human AFP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
Interference observed with other substances.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 2.89 | 6.02 | 12.3 | 2.65 | 5.62 | 11.7 |
| Standard Deviation | 0.109 | 0.219 | 0.441 | 0.158 | 0.261 | 0.549 |
| CV% | 3.8 | 3.6 | 3.6 | 6.0 | 4.6 | 4.7 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 3.15 | 6.62 | 13.2 | 3.22 | 6.79 | 14.1 |
| Standard Deviation | 0.080 | 0.149 | 0.447 | 0.215 | 0.382 | 0.930 |
| CV% | 2.5 | 2.3 | 3.4 | 6.7 | 5.6 | 6.6 |
Recovery for Human alpha-Fetoprotein Quantikine ELISA Kit
The recovery of AFP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 95-103 |
| EDTA Plasma (n=4) | 104 | 94-114 |
| Heparin Plasma (n=4) | 103 | 95-115 |
| Serum (n=4) | 103 | 86-115 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of AFP were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human alpha-Fetoprotein Quantikine ELISA Kit
Human alpha-Fetoprotein/AFP ELISA Calibrator Diluent RD5-24 Standard Curve
Human alpha-Fetoprotein/AFP ELISA Calibrator Diluent RD5-33 Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: alpha-Fetoprotein/AFP
Additional alpha-Fetoprotein/AFP Products
Product Documents for Human alpha-Fetoprotein Quantikine ELISA Kit
Product Specific Notices for Human alpha-Fetoprotein Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.