Human Amyloid beta (aa1-40) ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DAB140B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Tissue Lysates (10 µL), Cerebrospinal Fluid (10 µL)
Sensitivity
8.17 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid)
Product Summary for Human Amyloid beta (aa1-40) Quantikine ELISA Kit
The Quantikine® Human Amyloid beta (aa1-40) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Amyloid beta (aa1-40) in cell culture supernates, tissue lysates, and cerebrospinal fluid. It contains synthetic human Amyloid beta (aa1-40) and antibodies raised against the synthetic peptide. Results obtained for naturally occurring human Amyloid beta (aa1-40) showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-40).
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural human Amyloid beta (aa1-40).
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cerebrospinal Fluid, Tissue Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 58.9 | 264 | 556 | 56.3 | 247 | 518 |
| Standard Deviation | 4.23 | 5.76 | 11.3 | 4.31 | 22.4 | 30.5 |
| CV% | 7.2 | 2.2 | 2.0 | 7.7 | 9.1 | 5.9 |
Recovery for Human Amyloid beta (aa1-40) Quantikine ELISA Kit
The recovery of human Amyloid beta spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 95-105 |
| Cell Lysis Buffer (n=2) | 99 | 91-110 |
| Cerebrospinal Fluid (n=4) | 104 | 96-113 |
Linearity
To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Amyloid beta (aa1-40) Quantikine ELISA Kit
Human Amyloid beta (aa 1-40) ELISA Standard Curve
Human Amyloid beta (aa 1-40) ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Amyloid beta
Alternate Names
Abeta, beta-Amyloid Peptide
Gene Symbol
APP
Additional Amyloid beta Products
Product Documents for Human Amyloid beta (aa1-40) Quantikine ELISA Kit
Product Specific Notices for Human Amyloid beta (aa1-40) Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.