Human BMP-7 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DBP700
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Bone Extracts (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (50 µL)
Sensitivity
7.83 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Bone Extracts, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human BMP-7 Quantikine ELISA Kit
The Quantikine Human BMP-7 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BMP-7 in bone tissue extracts, cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human BMP-7 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BMP-7 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BMP-7.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human BMP-7
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Bone Extracts, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 365 | 783 | 1194 | 336 | 718 | 1110 |
| Standard Deviation | 22.1 | 35.9 | 80.7 | 31.9 | 64.9 | 86.4 |
| CV% | 6.1 | 4.6 | 6.8 | 9.5 | 9.0 | 7.8 |
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 370 | 814 | 1241 | 356 | 752 | 1148 |
| Standard Deviation | 20.6 | 26.8 | 40.9 | 27.5 | 54.5 | 81.5 |
| CV% | 5.6 | 3.3 | 3.3 | 7.7 | 7.2 | 7.1 |
Recovery for Human BMP-7 Quantikine ELISA Kit
The recovery of BMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Bone Extraction Solution (n=2) | 96 | 88-102 |
| Cell Culture Media (n=4) | 98 | 90-114 |
| EDTA Plasma (n=4) | 100 | 93-105 |
| Heparin Plasma (n=4) | 106 | 96-115 |
| Serum (n=4) | 100 | 90-105 |
| Urine (n=4) | 97 | 90-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of BMP-7 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human BMP-7 Quantikine ELISA Kit
Human BMP-7 ELISA Cell Culture Supernate/Urine Standard Curve
Human BMP-7 ELISA Serum/Plasma/Bone Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BMP-7
BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.
Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.
Long Name
Bone Morphogenetic Protein 7
Alternate Names
BMP7, OP-1
Gene Symbol
BMP7
Additional BMP-7 Products
Product Documents for Human BMP-7 Quantikine ELISA Kit
Product Specific Notices for Human BMP-7 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.