Human C-Peptide Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DICP00
Key Product Details
Assay Length
2.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (10 µL)
Sensitivity
2.88 pmol/L
Assay Range
28.9-1850 pmol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human C-Peptide Quantikine ELISA Kit
The Quantikine Human Insulin C-Peptide Immunoassay is a 2.5 hour solid-phase ELISA designed to measure human Insulin C-Peptide in cell culture supernates, serum, plasma, and urine. It contains synthetic human Insulin C-Peptide and has been shown to accurately quantitate the peptide. Results obtained using natural human Insulin C-Peptide showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Insulin C-Peptide.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and synthetic human Insulin C-Peptide.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pmol/L) | 129 | 436 | 845 | 127 | 437 | 861 |
| Standard Deviation | 5.77 | 14.3 | 13.2 | 12.4 | 33.0 | 65.3 |
| CV% | 4.5 | 3.3 | 1.6 | 9.8 | 7.6 | 7.6 |
Recovery for Human C-Peptide Quantikine ELISA Kit
The recovery of human Insulin C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 84-106 |
| EDTA Plasma (n=4) | 101 | 95-108 |
| Heparin Plasma (n=4) | 102 | 90-115 |
| Serum (n=4) | 103 | 96-109 |
| Urine (n=4) | 97 | 81-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Insulin C-Peptide were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human C-Peptide Quantikine ELISA Kit
Human C-Peptide ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: C-Peptide
Long Name
Proinsulin C-Peptide
Alternate Names
C-P, Insulin C-Peptide, Proinsulin C-Peptide
Gene Symbol
INS
Additional C-Peptide Products
Product Documents for Human C-Peptide Quantikine ELISA Kit
Product Specific Notices for Human C-Peptide Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.