Human Calbindin D Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCALD0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Tissue Lysates (10 µL), Urine (25 µL)
Sensitivity
0.015 ng/mL
Assay Range
0.078-5 ng/mL
Product Summary for Human Calbindin D Quantikine ELISA Kit
The Quantikine Human Calbindin D Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Calbindin D in tissue lysates and urine. It contains E. coli-expressed recombinant human Calbindin D and antibodies raised against the recombinant factor. Natural human Calbindin D showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural human Calbindin D.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Calbindin D.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Tissue Lysates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.511 | 1.65 | 2.84 | 0.548 | 1.57 | 2.96 |
| Standard Deviation | 0.026 | 0.040 | 0.072 | 0.038 | 0.075 | 0.150 |
| CV% | 5.1 | 2.4 | 2.5 | 6.9 | 4.8 | 5.1 |
Recovery for Human Calbindin D Quantikine ELISA Kit
The recovery of human Calbindin D spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Urine (n=4) | 93 | 89-98 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human Calbindin D were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Human Calbindin D Quantikine ELISA Kit
Human Calbindin D ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Calbindin D
Calbindin D is a highly conserved vitamin D-dependent cytoplasmic protein in the EF-hand calcium binding protein family that includes calmodulin, parvalbumin, troponin C, and S100. Calbindin-D is widely expressed and regulates calcium homeostasis. Calbindin-D protects neurons from excitotoxic and apoptotic cell death by buffering excess calcium.
Long Name
Vitamin D-dependent Calcium-Binding Protein
Alternate Names
CABP, CABP1, CABP28K, CABP9K, Calbindin 3, Calbindin-D, Calbindin-D9K, S100G
Gene Symbol
S100G
Additional Calbindin D Products
Product Documents for Human Calbindin D Quantikine ELISA Kit
Product Specific Notices for Human Calbindin D Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.