Human Carbonic Anhydrase IX Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCA900
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL), Urine (100 µL)
Sensitivity
4.39 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Product Summary for Human Carbonic Anhydrase IX Quantikine ELISA Kit
The Quantikine Human Carbonic Anhydrase IX Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CA9 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human CA9 ectodomain and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CA9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CA9.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human CA9
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 166 | 338 | 673 | 166 | 333 | 647 |
| Standard Deviation | 6.36 | 7.19 | 20.5 | 10.5 | 21.6 | 39.7 |
| CV% | 3.8 | 2.1 | 3.0 | 6.3 | 6.5 | 6.1 |
Recovery for Human Carbonic Anhydrase IX Quantikine ELISA Kit
The recovery of CA9 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 110 | 103-114 |
| Citrate Plasma (n=4) | 96 | 89-111 |
| EDTA Plasma (n=4) | 99 | 90-110 |
| Heparin Plasma (n=4) | 97 | 88-112 |
| Serum (n=4) | 97 | 87-106 |
| Urine (n=4) | 101 | 89-114 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CA9 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Carbonic Anhydrase IX Quantikine ELISA Kit
Human Carbonic Anhydrase IX/CA9 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Carbonic Anhydrase IX/CA9
Additional Carbonic Anhydrase IX/CA9 Products
Product Documents for Human Carbonic Anhydrase IX Quantikine ELISA Kit
Product Specific Notices for Human Carbonic Anhydrase IX Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.