Human CD25/IL-2R alpha ELISA Kit - Quantikine
R&D Systems, part of Bio-Techne | Catalog # DR2A00
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL)
Sensitivity
10 pg/mL
Assay Range
78-5000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human CD25/IL-2R alpha Quantikine ELISA Kit
The Quantikine Human IL-2 sR alpha Immunoassay is a 3.5 hour solid phase ELISA designed to measure IL-2 sR alpha in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human IL-2 sR alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-2 sR alpha showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2 sR alpha.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-2 sR alpha
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human IL-2 R alpha in this assay. No medical histories were available for the donors used in this
study.
Cell Culture Supernates - Human peripheral blood mononuclear cells (1 x 106 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cell were cultured unstimulated or stimulated with 10 μg/mL of PHA for 1, 3, and 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human IL-2 Ra.
| Sample Type | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=55) | 1055 | 458-1997 | 365 |
| EDTA plasma (n=55) | 1062 | 410-2623 | 424 |
| Heparin plasma (n=55) | 1020 | 430-2082 | 365 |
Cell Culture Supernates - Human peripheral blood mononuclear cells (1 x 106 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cell were cultured unstimulated or stimulated with 10 μg/mL of PHA for 1, 3, and 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human IL-2 Ra.
| Condition | Day 1 | Day 3 | Day 5 |
| Unstimulated | 245 | 459 | 645 |
| Stimulated | 3831 | 10,001 | 7765 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 207 | 613 | 2357 | 188 | 630 | 2615 |
| Standard Deviation | 12.7 | 37.5 | 108 | 13.6 | 38.1 | 183 |
| CV% | 6.1 | 6.1 | 4.6 | 7.2 | 6.0 | 7.0 |
Recovery for Human CD25/IL-2R alpha Quantikine ELISA Kit
The recovery of IL-2 sR alpha spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 105 | 93-118 |
| EDTA Plasma (n=5) | 103 | 91-113 |
| Heparin Plasma (n=5) | 104 | 98-113 |
| Serum (n=5) | 105 | 99-116 |
Linearity
To assess the linearity of the assay, five samples containing or spiked with high concentrations of IL-2 sR alpha in various matrices were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CD25/IL-2R alpha Quantikine ELISA Kit
Human IL2RA ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD25/IL-2R alpha
Long Name
Interleukin 2 Receptor alpha
Alternate Names
CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA
Entrez Gene IDs
Gene Symbol
IL2RA
Additional CD25/IL-2R alpha Products
Product Documents for Human CD25/IL-2R alpha Quantikine ELISA Kit
Product Specific Notices for Human CD25/IL-2R alpha Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.