Human CD30/TNFRSF8 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY6126-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
93.8-6000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human CD30/TNFRSF8 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human CD30/TNFRSF8 DuoSet ELISA
Human CD30 / TNFRSF8 ELISA Standard Curve
Kit Contents for Human CD30/TNFRSF8 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD30/TNFRSF8
CD30/TNFRSF8 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. The ligand for CD30 is CD30 Ligand/TNFSF8 (CD153), a member of the TNF superfamily. CD30 ligation by CD30 Ligand mediates pleiotropic effects, including cell proliferation, activation, differentiation and apoptosis.
Alternate Names
CD30, TNFRSF8
Gene Symbol
TNFRSF8
Additional CD30/TNFRSF8 Products
Product Documents for Human CD30/TNFRSF8 DuoSet ELISA
Product Specific Notices for Human CD30/TNFRSF8 DuoSet ELISA
For research use only
Loading...
Loading...
Loading...