Human Chemerin Quantikine ELISA
R&D Systems, part of Bio-Techne | Catalog # DCHM00
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
7.8 pg/mL
Assay Range
78-5000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human Chemerin Quantikine ELISA
The Quantikine Human Chemerin Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Chemerin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Chemerin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Chemerin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Chemerin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Chemerin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 513 | 1558 | 3016 | 515 | 1592 | 3147 |
| Standard Deviation | 23.3 | 66.5 | 84.6 | 40.5 | 121 | 200 |
| CV% | 4.5 | 4.3 | 2.8 | 7.9 | 7.6 | 6.4 |
Recovery for Human Chemerin Quantikine ELISA
The recovery of Chemerin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 104 | 94-111 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Chemerin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Chemerin Quantikine ELISA
Human Chemerin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Chemerin
Long Name
Retinoic Acid Receptor Responder Protein 2
Alternate Names
RARRES2, TIG-2
Gene Symbol
RARRES2
Additional Chemerin Products
Product Documents for Human Chemerin Quantikine ELISA
Product Specific Notices for Human Chemerin Quantikine ELISA
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.