Human Clusterin Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DCLU00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (25 µL), Urine (13 µL)
Sensitivity
1.05 ng/mL
Assay Range
3.12-200 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human Clusterin Quantikine ELISA Kit
The Quantikine Human Clusterin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Clusterin in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human Clusterin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Clusterin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Clusterin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Clusterin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Saliva/Urine - Samples from apparently healthy volunteers were evaluated for
the presence of human Clusterin in this assay. No medical histories were available for the
donors used in this study.
Cell Culture Supernates:
| Sample Type | Mean (μg/mL) | Range (μg/mL) | Standard Deviation (μg/mL) |
| Serum (n=36) | 266 | 195-378 | 42.8 |
| Heparin plasma (n=36) | 266 | 190-377 | 44.6 |
| EDTA plasma (n=36) | 261 | 185-436 | 52.0 |
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Salive (n=17) | 334 | 85.2-624 | 176 |
| Urine (n=21) | 280 | 85.2-628 | 171 |
Cell Culture Supernates:
Human peripheral blood leukocytes (PBLs) were cultured in DMEM supplemented with
5% fetal bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and
100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL
PHA. Aliquots of the cell culture supernates were removed and assayed for levels of human
Clusterin. All samples were non-detectable.
HepG2 human hepatocellular carcinoma cells were cultured in MEM (NEAA salts)
supplemented with 10% fetal bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine,
100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture
supernate was removed, assayed for human Clusterin, and measured 574 ng/mL.
HeLa cervical epithelial carcinoma cells were cultured in RPMI supplemented with 10% fetal
bovine serum, 50 μM beta-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and
100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed
for human Clusterin, and measured 863 ng/mL.
HUVEC human umbilical vein endothelial cells were cultured in EGM-2 media and stimulated
with recombinant human IL-1 beta for 24 hours. An aliquot of the cell culture supernate was
removed, assayed for human Clusterin, and measured 17.3 ng/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 19.0 | 64.3 | 130 | 22.1 | 64.1 | 136 |
| Standard Deviation | 0.7 | 2.4 | 4.5 | 1.6 | 5.4 | 9.3 |
| CV% | 3.7 | 3.7 | 3.5 | 7.2 | 8.4 | 6.8 |
Recovery for Human Clusterin Quantikine ELISA Kit
The recovery of Clusterin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 107 | 102-110 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of Clusterin were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Clusterin Quantikine ELISA Kit
Human Clusterin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Clusterin
High μg/mL concentrations of Clusterin circulate predominantly as a component of high
density lipoprotein particles, and these are internalized and degraded through interactions
with LRP-2/Megalin (10, 11). The ability of Clusterin to bind and neutralize non-oxidatively
modified LDL reduces cytotoxicity in atherosclerotic plaques (12). The chaperone function of
Clusterin helps to reduce the accumulation of beta-amyloid fibrils and damage due to amyloid
plaques in Alzheimer's disease (13). An alternately spliced 50 kDa isoform of human Clusterin
(nCLU) remains intracellular and is neither glycosylated nor cleaved into alpha and beta chains (5, 14).
Cellular exposure to ionizing radiation promotes the translocation of nCLU to the nucleus
where it interacts with Ku70 and promotes apoptosis (5, 14). This function contrasts with the
cytoprotective effect of secreted Clusterin (15). During tumor progression, nCLU is down
regulated while the secreted form is upregulated and may be aberrantly glycosylated (14, 16,
17). Increased circulating levels of Clusterin enhance tumor aggressiveness by inhibiting
apoptosis and by promoting the epithelial to mesenchymal transition (18-20).
Alternate Names
APOJ, CLI, CLU, SGP-2, SP-40, TRPM-2
Gene Symbol
CLU
Additional Clusterin Products
Product Documents for Human Clusterin Quantikine ELISA Kit
Product Specific Notices for Human Clusterin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.