Human CXCL1/GRO alpha Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DGR00B
Key Product Details
Assay Length
4.25-4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (200 µL), Platelet-poor EDTA Plasma (200 µL), Platelet-poor Heparin Plasma (200 µL), Platelet-poor Citrate Plasma (200 µL)
Sensitivity
10 pg/mL
Assay Range
31.3-1000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Platelet-poor Citrate Plasma)
Product Summary for Human CXCL1/GRO alpha Quantikine ELISA Kit
The Quantikine® Human CXCL1/GRO alpha Immunoassay is a 4.25-4.5 hour solid phase ELISA designed to measure human GRO alpha in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human GRO alpha and antibodies raised against the recombinant protein. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human GRO alpha showed linear curves that were parallel to the
standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human GRO alpha.
standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human GRO alpha.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human GRO alpha.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 41.8 | 140 | 373 | 48.7 | 157 | 379 |
| Standard Deviation | 0.91 | 5.98 | 15.7 | 2.90 | 12.3 | 29.8 |
| CV% | 2.2 | 4.3 | 4.2 | 6.0 | 7.8 | 7.8 |
Platelet-poor Citrate Plasma, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 61.4 | 251 | 736 | 62.8 | 252 | 734 |
| Standard Deviation | 2.2 | 6.0 | 20.0 | 3.6 | 11.9 | 38.9 |
| CV% | 3.6 | 2.4 | 2.7 | 5.7 | 4.7 | 5.3 |
Recovery for Human CXCL1/GRO alpha Quantikine ELISA Kit
The recovery of human GRO alpha spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 99 | 92-105 |
| Platelet-poor Citrate Plasma (n=4) | 90 | 80-98 |
| Platelet-poor EDTA Plasma (n=4) | 93 | 86-102 |
| Platelet-poor Heparin Plasma (n=4) | 95 | 83-109 |
| Serum (n=4) | 98 | 87-108 |
Linearity
To assess linearity of the assay, samples containing and/or spiked with high concentrations of human GRO alpha were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CXCL1/GRO alpha Quantikine ELISA Kit
Human CXCL1/GRO alpha/KC/CINC-1 ELISA Cell Culture Supernate Standard Curve
Human GRO alpha ELISA Cell Culture Supernate Standard Curve
Human GRO alpha ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL1/GRO alpha/KC/CINC-1
Alternate Names
CINC-1, CINC1, GRO alpha, KC, MGSA-alpha
Gene Symbol
CXCL1
Additional CXCL1/GRO alpha/KC/CINC-1 Products
Product Documents for Human CXCL1/GRO alpha Quantikine ELISA Kit
Product Specific Notices for Human CXCL1/GRO alpha Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.