Human CXCL12/SDF-1 alpha Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DSA00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Platelet-poor EDTA Plasma (100 µL), Platelet-poor Heparin Plasma (100 µL)
Sensitivity
47 pg/mL
Assay Range
156-10000 pg/mL (Cell Culture Supernates, EDTA Plasma, Platelet-poor Heparin Plasma)
Product Summary for Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
The Quantikine Human SDF-1 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SDF-1 alpha in cell culture supernates and platelet-poor plasma. It contains E. coli-expressed recombinant human SDF-1 alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human SDF-1 alpha. Results obtained using natural SDF-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural SDF-1 alpha.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant SDF-1 alpha
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 491 | 1700 | 3420 | 544 | 1600 | 3150 |
| Standard Deviation | 19 | 58 | 115 | 73 | 150 | 257 |
| CV% | 3.9 | 3.4 | 3.4 | 13.4 | 9.4 | 8.2 |
Recovery for Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
The recovery of SDF-1 alpha spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 95-108 |
| Platelet-poor EDTA Plasma (n=5) | 97 | 90-107 |
| Platelet-poor Heparin Plasma (n=5) | 97 | 90-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SDF-1 alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
Human CXCL12/SDF-1 alpha ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL12/SDF-1 alpha
Alternate Names
SDF1 alpha, SDF1a
Gene Symbol
CXCL12
Additional CXCL12/SDF-1 alpha Products
Product Documents for Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
Product Specific Notices for Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.