Human DPPIV/CD26 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DC260B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (50 µL)
Sensitivity
0.072 ng/mL
Assay Range
0.313-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human DPPIV/CD26 Quantikine ELISA Kit
The Quantikine® Human DPPIV/CD26 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human DPPIV in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human DPPIV, and antibodies raised against the recombinant factor. Results obtained using natural human DPPIV showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human DPPIV.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Reactivity
Human
Specificity
Natural and recombinant human DPPIV.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 2.16 | 8.15 | 14.1 | 2.22 | 8.25 | 14.6 |
| Standard Deviation | 0.105 | 0.280 | 0.916 | 0.140 | 0.695 | 1.18 |
| CV% | 4.9 | 3.4 | 6.5 | 6.3 | 8.4 | 8.1 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 2.32 | 8.78 | 15.8 | 2.43 | 8.53 | 15.9 |
| Standard Deviation | 0.142 | 0.354 | 1.15 | 0.207 | 0.777 | 1.30 |
| CV% | 6.1 | 4.0 | 7.3 | 8.5 | 9.1 | 8.2 |
Recovery for Human DPPIV/CD26 Quantikine ELISA Kit
The recovery of human DPPIV spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 87-113 |
| Urine (n=4) | 101 | 88-113 |
Linearity
To assess linearity of the assay, samples containing and/or spiked with high concentrations of human DPPIV were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human DPPIV/CD26 Quantikine ELISA Kit
Human DPPIV/CD26 ELISA Standard Curve
Human DPPIV/CD26 ELISA Standard Curve
Human DPPIV/CD26 ELISA Cell Culture Supernate/Saliva/Urine Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: DPPIV/CD26
Long Name
Dipeptidyl-peptidase IV
Alternate Names
CD26, DPP4
Gene Symbol
DPP4
Additional DPPIV/CD26 Products
Product Documents for Human DPPIV/CD26 Quantikine ELISA Kit
Product Specific Notices for Human DPPIV/CD26 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.