Human EDA2R/TNFRSF27/XEDAR DuoSet ELISA

Name: Human EDA2R/TNFRSF27/XEDAR DuoSet ELISA
Brand: R&D Systems
SKU: DY1093

R&D Systems, part of Bio-Techne | Catalog # DY1093

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Product Summary
Product Specifications
Scientific Data
Kit Contents
Other Reagents Required
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

15.6-1000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human EDA2R/TNFRSF27/XEDAR DuoSet ELISA Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

View all EDA2R/TNFRSF27/XEDAR ELISA Kits »

Product Summary for Human EDA2R/TNFRSF27/XEDAR DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human EDA2R / TNFRSF27 / XEDAR. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human EDA2R/TNFRSF27/XEDAR DuoSet ELISA

Human EDA2R / TNFRSF27 / XEDAR ELISA Standard Curve

Kit Contents for Human EDA2R/TNFRSF27/XEDAR DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).

Blocking Buffer:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EDA2R/TNFRSF27/XEDAR

EDA2R, also known as TNFRSF27 or XEDAR (X-linked ectodysplasin receptor), is an X-linked member of the TNF receptor superfamily (TNFRSF). Within the TNFRSF, EDA2R shares the highest homologies with EDAR and TNFRSF19/TROY. EDA-A2 is the EDA2R ligand. EDA2R expression is principally found in embryonic hair follicles. EDA2R, EDAR, EDA-A1, and EDA-A2 have been associated with hypohidrotic ectodermal dysplasia (HED).

Long Name

Ectodysplasin A2 Receptor

Alternate Names

EDAA2R, TNFRSF27, XEDAR

Entrez Gene IDs

60401 (Human); 245527 (Mouse); 710244 (Rhesus Macaque)

Gene Symbol

EDA2R

Additional EDA2R/TNFRSF27/XEDAR Products

Product Documents for Human EDA2R/TNFRSF27/XEDAR DuoSet ELISA

Product Datasheets

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COA

SDS

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Product Specific Notices for Human EDA2R/TNFRSF27/XEDAR DuoSet ELISA

For research use only

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