Human EGF ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DEG00
Key Product Details
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (10 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL), Platelet-poor Citrate Plasma (200 µL), Urine (10 µL)
Sensitivity
0.7 pg/mL
Assay Range
3.9-250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma, Urine)
Product Summary for Human EGF Quantikine ELISA Kit
The Quantikine Human EGF Immunoassay kit is a 3.5 or 4.5 hour solid phase ELISA designed to measure EGF levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human EGF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human EGF. Results obtained measuring natural human EGF showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural EGF.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human EGF
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Saliva/Urine - Samples from apparently healthy volunteers were evaluated for
the presence of human EGF in this assay. No medical histories were available for the donors
used in this study.
ND=Non-detectable
Cell Culture Supernates - - Human peripheral blood leukocytes (PBL; 1 x 106 cells) were cultured in RPMI 1640 and supplemented with 10% fetal bovine serum. Cells were cultured stimulated with 10 μg/mL PHA for 6 days. An aliquot of the cell culture supernate was removed, assayed for human EGF, and measured 5.4 pg/mL.
| Sample Type | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Platelet-poor EDTA plasma (n=37) | 4.87 | 5 | 4.54-5.20 |
| Platelet-poor heparin plasma (n=37) | 4.81 | 16 | ND-5.43 |
| Platelet-poor citrate plasma (n=37) | ND | 0 | _____ |
| Sample Type | Mean (pg/mL) | Range (pg/mL) | Standard Deviation (pg/mL) |
| Serum (n=37) | 409 | 106-1063 | 151 |
| Saliva (n=14) | 1703 | 81.8-9600 | 2400 |
| Sample Type | Mean (μg/g Creatinine) | Range (μg/g Creatinine) | Standard Deviation (μg/g Creatinine) |
| Urine (n=11) | 23,019 | 12,352-36,894 | 8524 |
Cell Culture Supernates - - Human peripheral blood leukocytes (PBL; 1 x 106 cells) were cultured in RPMI 1640 and supplemented with 10% fetal bovine serum. Cells were cultured stimulated with 10 μg/mL PHA for 6 days. An aliquot of the cell culture supernate was removed, assayed for human EGF, and measured 5.4 pg/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 15.0 | 78.9 | 134 | 15.0 | 80.0 | 136 |
| Standard Deviation | 0.4 | 2.5 | 4.0 | 0.9 | 4.5 | 6.8 |
| CV% | 2.7 | 3.2 | 3.0 | 6.0 | 5.6 | 5.0 |
EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 13.5 | 80 | 133 | 14.7 | 77 | 134 |
| Standard Deviation | 0.6 | 2.0 | 2.7 | 0.9 | 3.3 | 6.1 |
| CV% | 4.4 | 2.5 | 2.0 | 6.1 | 4.3 | 4.6 |
Recovery for Human EGF Quantikine ELISA Kit
The recovery of EGF spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 98 | 88-111 |
| Citrate Plasma (n=10) | 104 | 89-115 |
| EDTA Plasma (n=10) | 100 | 80-111 |
| Heparin Plasma (n=10) | 96 | 79-108 |
| Serum (n=10) | 97 | 80-106 |
| Urine (n=1) | 102 | 97-108 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of EGF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human EGF Quantikine ELISA Kit
Human EGF ELISA Cell Culture Supernate/Urine Standard Curve
Human EGF ELISA Serum/Plasma/Saliva Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: EGF
Long Name
Epidermal Growth Factor
Alternate Names
HOMG4, URG, Urogastrone
Gene Symbol
EGF
Additional EGF Products
Product Documents for Human EGF Quantikine ELISA Kit
Product Specific Notices for Human EGF Quantikine ELISA Kit
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.