Human EMMPRIN/CD147 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DEMP00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (50 µL), Urine (10 µL), Human Milk (10 µL)
Sensitivity
9.77 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Product Summary for Human EMMPRIN/CD147 Quantikine ELISA Kit
The Quantikine Human EMMPRIN Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human EMMPRIN in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human EMMPRIN and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human EMMPRIN showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally ocurring EMMPRIN.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human EMMPRIN
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Human Milk, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 448 | 1257 | 2412 | 396 | 1183 | 2363 |
| Standard Deviation | 20.1 | 43.0 | 120 | 21.1 | 70.5 | 120 |
| CV% | 4.5 | 3.4 | 5 | 5.3 | 6.0 | 5.1 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 431 | 1208 | 2341 | 421 | 1240 | 2474 |
| Standard Deviation | 15.3 | 46.9 | 109 | 23.7 | 54.9 | 145 |
| CV% | 3.5 | 3.9 | 4.7 | 5.6 | 4.4 | 5.9 |
Recovery for Human EMMPRIN/CD147 Quantikine ELISA Kit
The recovery of EMMPRIN spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 91-111 |
| EDTA Plasma (n=4) | 98 | 90-110 |
| Heparin Plasma (n=4) | 96 | 85-104 |
| Serum (n=4) | 100 | 90-111 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of EMMPRIN were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human EMMPRIN/CD147 Quantikine ELISA Kit
Human EMMPRIN/CD147 ELISA Serum/Plasma Standard Curve
Human EMMPRIN/CD147 ELISA Cell Culture Supernates/Saliva/Urine/Human Milk Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: EMMPRIN/CD147
Long Name
Extracellular Matrix Metalloproteinase Inducer
Alternate Names
Basigin, BSG, CD147
Gene Symbol
BSG
Additional EMMPRIN/CD147 Products
Product Documents for Human EMMPRIN/CD147 Quantikine ELISA Kit
Product Specific Notices for Human EMMPRIN/CD147 Quantikine ELISA Kit
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.