Human FLRG Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DFLRG0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL), Urine (50 µL), Human Milk (50 µL)
Sensitivity
8.55 pg/mL
Assay Range
62.5-4000 pg/mL
Product Summary for Human FLRG Quantikine ELISA Kit
The Quantikine Human FLRG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FLRG in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human FLRG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FLRG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FLRG.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human FLRG
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 468 | 1113 | 2252 | 494 | 1174 | 2331 |
| Standard Deviation | 10.4 | 23.0 | 55.2 | 32.9 | 69.0 | 143 |
| CV% | 2.2 | 2.1 | 2.5 | 6.7 | 5.9 | 6.1 |
EDTA Plasma, Heparin Plasma, Human Milk, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 448 | 1094 | 2306 | 486 | 1148 | 2282 |
| Standard Deviation | 15.4 | 18.5 | 49.5 | 29.5 | 53.0 | 122 |
| CV% | 3.4 | 1.7 | 2.1 | 6.1 | 4.6 | 5.3 |
Recovery for Human FLRG Quantikine ELISA Kit
The recovery of FLRG spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 107 | 98-111 |
| EDTA Plasma (n=4) | 96 | 85-105 |
| Heparin Plasma (n=4) | 102 | 89-114 |
| Serum (n=4) | 100 | 91-112 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of FLRG were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human FLRG Quantikine ELISA Kit
Human FLRG ELISA Serum/Plasma/Human Milk Standard Curve
Human FLRG ELISA Cell Culture Supernate/Urine Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Follistatin-related Gene Protein/FLRG
Alternate Names
Follistatin-like 3, FSTL3
Gene Symbol
FSTL3
Additional Follistatin-related Gene Protein/FLRG Products
Product Documents for Human FLRG Quantikine ELISA Kit
Product Specific Notices for Human FLRG Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.