Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DGBP30B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (10 µL)
Sensitivity
0.161 ng/mL
Assay Range
0.391-25 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit
The Quantikine Human Galectin-3BP/MAC-2BP Immunoassay is a 4.5 hour solid phase ELISA
designed to measure Galectin-3BP in cell culture supernates, serum, plasma,
saliva, and urine. It contains NS0-expressed recombinant
human Galectin-3BP and antibodies raised against the recombinant factor. This
immunoassay has been shown to accurately quantitate recombinant human Galectin-3BP. Results obtained using natural human Galectin-3BP showed linear curves
that were parallel to the standard curves obtained using the Quantikine
kit standards. These results indicate that this kit can be used to
determine relative mass values for natural human Galectin-3BP.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Galectin-3BP/MAC-2BP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 32 | 32 | 32 |
| Mean (pg/mL) | 0.743 | 2.94 | 8.27 | 0.778 | 3.2 | 9.10 |
| Standard Deviation | 0.027 | 0.080 | 0.244 | 0.078 | 0.225 | 0.434 |
| CV% | 3.6 | 2.7 | 3.0 | 10.0 | 7.0 | 4.8 |
Recovery for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit
The recovery of human Galectin-3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 99 | 95-101 |
| Saliva (n=4) | 91 | 82-101 |
| Urine (n=4) | 104 | 98-111 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of Galectin-3BP were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit
Human Galectin-3BP/MAC-2BP ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Galectin-3BP/MAC-2BP
Long Name
Galectin-3 Binding Protein
Alternate Names
90K, Galectin3BP, LGALS3BP, MAC-2BP
Entrez Gene IDs
3959 (Human)
Gene Symbol
LGALS3BP
Additional Galectin-3BP/MAC-2BP Products
Product Documents for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit
Product Specific Notices for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.