Human Granzyme B Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DGZB00
Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL)
Sensitivity
4.8 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates)
Product Summary for Human Granzyme B Quantikine ELISA Kit
The Quantikine® Human Granzyme B Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Granzyme B in culture supernates. It contains NS0-expressed recombinant human Granzyme B and antibodies raised against the recombinant protein. Results obtained using natural human Granzyme B showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Granzyme B
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
natural and recombinant human Granzyme B.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 312 | 630 | 1298 | 311 | 615 | 1208 |
| Standard Deviation | 8.04 | 11.0 | 28.7 | 27.5 | 56.1 | 77.2 |
| CV% | 2.6 | 1.7 | 2.2 | 8.8 | 9.1 | 6.4 |
Recovery for Human Granzyme B Quantikine ELISA Kit
The recovery of human Granzyme B spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 93-114 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Granzyme B were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Granzyme B Quantikine ELISA Kit
Human Granzyme B Quantikine ELISA Kit Linearity Competitor Comparison
Human Granzyme B Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Granzyme B
As one of the five Granzymes (A, B, H, K and M) identified in the human genome, Granzyme B
(32kDa) (5) is the most widely researched in terms of its biological function and its utility in
health and disease (4). It is synthesized as a precursor (247 residues) with a signal peptide
(residues 1-18), a pro-peptide (residues 19-20), and a mature chain (residues 21-247) (6-8).
Once inside granules, Granzyme B is fully processed into the mature chain and becomes an
active protease when the pro-peptide, Gly-Glu is removed from the N-terminus by cleavage
with Cathepsin C (9). The protease activity of Granzyme B is tightly controlled by Serpin B9/
Protease Inhibitor 9 (9). The amino acid sequence of human Granzyme B is 71%, 69%, and 68%
identical to its canine, rat, and mouse counterparts, respectively.
Granzymes have been shown to modulate inflammation, and Granzyme B plasma levels have
been found higher with atopic dermatitis and psoriasis when compared to healthy controls.
This is in contrast to Granzyme A plasma levels which remain unchanged (10). Serum from
patients with Crohn's disease have significantly higher Granzyme B levels than controls (11).
Alternate Names
CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT
Gene Symbol
GZMB
Additional Granzyme B Products
Product Documents for Human Granzyme B Quantikine ELISA Kit
Product Specific Notices for Human Granzyme B Quantikine ELISA Kit
For research use only
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