Human HE4/WFDC2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DHE400
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Saliva (10 µL), Urine (10 µL)
Sensitivity
32.2 pg/mL
Assay Range
78.1-5000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human HE4/WFDC2 Quantikine ELISA Kit
The Quantikine Human HE4/WFDC2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human HE4/WFDC2 in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human HE4/WFDC2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human HE4/WFDC2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human HE4/WFDC2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human HE4/WFDC2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 470 | 1278 | 2448 | 476 | 1408 | 2731 |
| Standard Deviation | 11.5 | 35.3 | 86.1 | 26.0 | 79.1 | 162 |
| CV% | 2.4 | 2.8 | 3.5 | 5.5 | 5.6 | 5.9 |
Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 451 | 1337 | 2629 | 452 | 1409 | 2761 |
| Standard Deviation | 14.0 | 39.8 | 85.7 | 25.0 | 78.0 | 202 |
| CV% | 3.1 | 3.0 | 3.3 | 5.5 | 5.5 | 7.3 |
Recovery for Human HE4/WFDC2 Quantikine ELISA Kit
The recovery of HE4/WFDC2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 103 | 95-114 |
| EDTA Plasma (n=4) | 108 | 94-115 |
| Heparin Plasma (n=4) | 107 | 96-115 |
| Saliva (n=4) | 100 | 98-107 |
| Serum (n=4) | 104 | 92-115 |
| Urine (n=4) | 98 | 85-111 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of HE4/WFDC2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human HE4/WFDC2 Quantikine ELISA Kit
Human HE4/WFDC2 ELISA Cell Culture Supernate/Serum/Plasma/Saliva Standard Curve
Human HE4/WFDC2 ELISA Urine Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: HE4/WFDC2
Long Name
WAP Four-disulfide Core Domain protein 2
Alternate Names
EDDM4, WAP5, WFDC2
Gene Symbol
WFDC2
Additional HE4/WFDC2 Products
Product Documents for Human HE4/WFDC2 Quantikine ELISA Kit
Product Specific Notices for Human HE4/WFDC2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.