Human IFN-beta Quantikine QuicKit ELISA
R&D Systems, part of Bio-Techne | Catalog # QK410
Key Product Details
Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL)
Sensitivity
2.88 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates)
Product Summary for Human IFN-beta Quantikine QuicKit ELISA
The Quantikine™ QuicKit™ Human IFN-beta Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human IFN-beta levels in cell culture supernates. It contains CHO cell-expressed recombinant human IFN-beta and antibodies raised against the recombinant protein. Results obtained using natural human IFN-beta showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-beta.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IFN-beta.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Cell Culture Supernates:
PBMCs were isolated from a single donor over a Ficoll-Paque PLUS density gradient. PBMCs
were seeded at 5 x 106
/mL and cultured in RPMI 1640 supplemented with 10% fetal bovine
serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells
were left untreated or treated with 10 μg/mL of poly I:C in the presence of Lipofectamine 2000
for 24 hours. Aliquots of the cell culture supernates were removed and assayed for levels of
human IFN-beta.
ND= Non-detectable
ND=Non-detectable
| Condition | pg/mL |
| Untreated | ND |
| Treated | 323 |
A549 adenocarcinomic human alveolar basal epithelial cells were cultured in Kaighn's Nutrient
Mixture F-12 supplemented with 10% fetal bovine serum until nearly confluent. The cells were
left untreated or treated with 10 μg/mL of poly I:C in the presence of Lipofectamine 2000 for
24 hours. Aliquots of the cell culture supernates were removed and assayed for levels of human
IFN-beta.
| Condition | pg/mL |
| Untreated | ND |
| Treated | 1470 |
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 101 | 613 | 121 | 669 |
| Standard Deviation | 3.82 | 30.1 | 6.48 | 33.3 |
| CV% | 3.8 | 4.9 | 5.4 | 5.0 |
Recovery for Human IFN-beta Quantikine QuicKit ELISA
The recovery of human IFN-beta spiked to three levels in samples throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Serum (n=0) |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human IFN-beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IFN-beta Quantikine QuicKit ELISA
Human IFN-beta Standard Curve ELISA
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-beta
Long Name
Interferon beta
Alternate Names
IFB, IFF, IFNB, IFNB1, IFNbeta
Gene Symbol
IFNB1
Additional IFN-beta Products
Product Documents for Human IFN-beta Quantikine QuicKit ELISA
Product Specific Notices for Human IFN-beta Quantikine QuicKit ELISA
For research use only
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