Human IGF-I/IGF-1 ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DG100B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (13 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.022 ng/mL
Assay Range
0.063-4 ng/mL (Serum-free Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human IGF-I/IGF-1 Quantikine ELISA Kit
The Quantikine® Human IGF-I/IGF-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IGF-1 in cell culture supernates, serum, and plasma. It contains E.
coli-expressed recombinant human IGF-1 and has antibodies raised againt the recombinant protein. Results obtained for naturally occurring human IGF-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IGF-1.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IGF-1.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human IGF-1 in this assay. No medical histories were available for the donors used in this
study.
Cell Culture Supernates:
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Serum (n=30) | 117 | 58.8-201 | 37.2 |
| EDTA plasma (n=30) | 119 | 58.6-211 | 37.4 |
| Heparin plasma (n=30) | 118 | 62.4-232 | 39.0 |
Cell Culture Supernates:
HepG2 human hepatocellular carcinoma cells were grown in MEM NEAA Earl's Salts
supplemented with 1 mM sodium pyruvate, 2 mM L-glutamine, 100 U/mL penicillin, and
100 μg/mL streptomycin sulfate until confluent (normoxia). For hypoxia, cells were incubated
for an additional 72 hours in a hypoxia workstation set to 1% O2
. The cell conditioned media
was taken off and centrifuged to remove any cells or debris. Aliquots of the cell culture
supernates were removed and assayed for human IGF-1.
| Condition | (ng/mL) |
| Normoxia | 2.52 |
| Hypoxia | 6.59 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
EDTA Plasma, Heparin Plasma, Serum, Serum-free Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.594 | 1.02 | 1.94 | 0.617 | 1.01 | 2.10 |
| Standard Deviation | 0.026 | 0.046 | 0.077 | 0.038 | 0.060 | 0.120 |
| CV% | 4.4 | 4.5 | 4.0 | 6.2 | 5.9 | 5.7 |
Recovery for Human IGF-I/IGF-1 Quantikine ELISA Kit
The recovery of human IGF-1 spiked to three different levels throughout the range of the assay was evaluated. Samples were diluted prior to assay as described in the Sample Preparation section.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 93 | 88-97 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IGF-1 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay as described in the Sample Preparation section.
Scientific Data Images for Human IGF-I/IGF-1 Quantikine ELISA Kit
Human IGF-I/IGF-1 Standard Curve
Human IGF-I ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGF-I/IGF-1
IGF-1 is a growth hormone that plays a role in a variety of
biological events. It is produced primarily by hepatocytes, serving an
endocrine function. It is also produced by many other cells, where it may act
in an autocrine or paracrine manner. It binds two tyrosine kinase receptors,
the IGF-1 receptor (IGF1R) and the insulin receptor, to initiate downstream
events like the AKT and PI3K signal transduction pathways. This triggers cell
proliferation and protects cells from apoptosis. IGF-1 also interacts with
seven IGF-binding proteins (IGFBP-1 through IGFBP7), influencing IGF-1 binding
to IGF1R and increasing IGF-1 half-life to closely regulate IGF-1 signaling.
For example, IGFBP-3 binds over 90% of the total IGF in serum in a complex of
IGF, IGFBP, and an acid-labile subunit. This ternary complex greatly stabilizes
IGF in the circulation, changing the half-life from minutes to hours. Proteases
also facilitate IGF-1R binding by cleaving IGFBPs to modify their affinity for
IGF or completely eliminate the IGFBP. The interactions of IGF, IGFBP, IGFBP
proteases, and IGF receptors are referred to as the IGF axis.
The IGF axis affects many primary physiological and
pathological processes, including development, growth, metabolic regulation,
tumorigenesis, atherosclerosis, and angiogenesis. Its ability to inhibit
apoptosis and stimulate cell growth and proliferation plays a significant role
in prenatal development, growth to adulthood, and metabolic control. Serum
levels of IGF-1 have been reported to increase from birth to puberty, followed
by a slow decline through adulthood. IGF-1 also induces amino acid uptake, protein
synthesis, and glucose utilization. In the brain, IGF-1 acts as a neurotrophic
factor to promote neurogenesis and neuronal survival. Exercise increases levels
of IGF-1 in blood serum, indicating it plays a role as a key mediator of
exercise-induced neurogenesis.
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Alternate Names
IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C
Gene Symbol
IGF1
Additional IGF-I/IGF-1 Products
Product Documents for Human IGF-I/IGF-1 Quantikine ELISA Kit
Product Specific Notices for Human IGF-I/IGF-1 Quantikine ELISA Kit
For research use only
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