Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DLA50
Key Product Details
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL), Citrate Plasma (200 µL)
Sensitivity
1 pg/mL
Assay Range
3.9-250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
The Quantikine Human IL-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 alpha in cell culture supernates, serum, and plasma. It contains recombinant human IL-1 alpha and antibodies raised against recombinant human IL-1 alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-1 alpha showed linear curves that were parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1 alpha.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Sample Values
Serum/Plasma - Forty samples from apparently healthy volunteers were evaluated for the
presence of human IL-1 alpha, and all had levels which fell below the lowest IL-1 alpha standard,
3.9 pg/mL. No medical histories were available for the donors used in this study.
Cell Culture Supernates - Human peripheral blood mononuclear cells (5 x 106
cells/mL) were
cultured in RPMI supplemented with 5% fetal bovine serum, 50 μM beta-mercaptoethanol,
2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were
cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates
were removed on days 1 and 5 and assayed for levels of human IL-1 alpha.
| Condition | Day 1 (pg/mL) | Day 5 (pg/mL) |
| Unstimulated | 85 | 23 |
| Stimulated | 1330 | 395 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 26.9 | 122 | 191 | 20.5 | 60.0 | 121 |
| Standard Deviation | 0.4 | 4.6 | 6.6 | 1.7 | 3.1 | 5.2 |
| CV% | 1.5 | 3.8 | 3.5 | 8.3 | 5.2 | 4.3 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 28.6 | 125 | 185 | 27.9 | 123 | 187 |
| Standard Deviation | 0.4 | 1.4 | 4.0 | 1.2 | 4.7 | 6.3 |
| CV% | 1.4 | 1.1 | 2.2 | 4.3 | 3.8 | 3.4 |
Recovery for Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
The recovery of IL-1 alpha spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 91-104 |
| Citrate Plasma (n=6) | 97 | 71-119 |
| EDTA Plasma (n=6) | 104 | 84-121 |
| Heparin Plasma (n=6) | 104 | 72-126 |
| Serum (n=6) | 107 | 74-127 |
Linearity
To assess the linearity of the assay, the following biological samples spiked with high concentrations of IL-1 alpha were diluted with the appropriate Calibrator Diluent and then assayed.
Scientific Data Images for Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
Human IL-1 alpha/IL-1F1 ELISA Calibrator Diluent RD5-5 Standard Curve
Human IL-1 alpha/IL-1F1 ELISA Calibrator Diluent RD6C Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 alpha/IL-1F1
Long Name
Interleukin 1 alpha
Alternate Names
BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM
Entrez Gene IDs
Gene Symbol
IL1A
Additional IL-1 alpha/IL-1F1 Products
Product Documents for Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
Product Specific Notices for Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.