Human IL-8/CXCL8 DuoSet ELISA Best Seller
R&D Systems, part of Bio-Techne | Catalog # DY208
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
31.2-2000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human IL-8/CXCL8 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human IL-8/CXCL8 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CXCL8/IL-8. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human IL-8/CXCL8 DuoSet ELISA
Human IL-8 / CXCL8 ELISA Standard Curve
Kit Contents for Human IL-8/CXCL8 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Block Buffer: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Reagent Diluent: 0.1% BSA, 0.05% Tween 20 in Tris-buffered Saline (20 mM Trizma base, 150 mM NaCI) pH 7.2-7.4, 0.2 μm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-8/CXCL8
Multiple isoforms of IL-8 are generated through both
alternative splicing and differential proteolytic cleavage. In humans,
alternative splicing generates an iso-form with an eleven aa substitution at
the C-terminus. Proteolytic processing results in N-terminal truncation of IL-8
and is likely a cell-specific event. For example, fibroblasts and endothelial
cells generate the 1-77 form by cleaving IL-8 following Glu21, while monocytes
and lymphocytes generate the 6-77 form by cleaving following Leu25. These
truncated forms generally show increased bioactivity, particularly through the
CXCR1 receptor. IL-8 can also undergo citrullination on Arg27 of the precursor,
a modification that increases its half-life and ability to induce leukocytosis.
A wide variety of cells secrete IL-8 including monocytes and neutrophils, fibroblasts
and keratinocytes, mast cells, visceral smooth muscle cells, dendritic cells,
type II great alveolar cells, and endothelial cells.
IL-8 bioactivity is mediated through two G-protein-coupled
receptors, termed CXCR1/IL-8 RA and CXCR2/IL-8 RB. CXCR1 is 45-50 kDa in size
and is used almost exclusively by IL-8. CXCR2 is 35-40 kDa in size and is used
by nearly all CXC chemokines. Both CXCR1 and CXCR2 constitutively associate
into functional homodimers. They can also heterodimerize, but these complexes
dissociate following IL-8 binding. CXCR2 responds to low concentrations of IL-8
and is principally associated with chemotaxis and MMP-9 release. CXCR1, in
contrast, responds to high concentrations of IL-8 and is associated with
respiratory burst and phospholipase D2 activation. Thus, CXCR2 ligation induces
leukocyte adhesion to activated vascular endothelium and migration to sites of
inflammation, while CXCR1 ligation primes neutrophil antimicrobial activity.
IL-8 can also form a complex with Serpin A1/alpha-1 Antitrypsin, and this
prevents IL-8 interaction with CXCR1.
In addition to its pro-inflammatory effects, IL-8 is
involved in angiogenesis and the pathogenesis of atherosclerosis and cancer. It
induces VEGF expression in vascular endothelial cells and functions as an
autocrine factor for EC growth and angiogenesis. It is upregulated in
atherosclerotic lesions and is elevated in the serum and cerebrospinal fluid
following myocardial infarction. In cancer, IL-8 promotes
epithelial-mesenchymal transition as well as tumor cell invasiveness and
metastasis.
Long Name
Interleukin 8
Alternate Names
CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1
Gene Symbol
CXCL8
Additional IL-8/CXCL8 Products
Product Documents for Human IL-8/CXCL8 DuoSet ELISA
Product Specific Notices for Human IL-8/CXCL8 DuoSet ELISA
For research use only
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