Human IL-9 DuoSet ELISA

Name: Human IL-9 DuoSet ELISA
Brand: R&D Systems
SKU: DY209-05
Rating: 4 (2 reviews)

R&D Systems, part of Bio-Techne | Catalog # DY209-05

(2)

Citations (10)

Product Datasheet / COA / SDS

100% Guarantee

Product Specifications
Scientific Data
Kit Contents
Other Reagents Required
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

39.1-2500 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human IL-9 DuoSet ELISA Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

View all IL-9 ELISA Kits »

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human IL-9 DuoSet ELISA

Human IL-9 ELISA Standard Curve

Kit Contents for Human IL-9 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-9

Mouse interleukin 9 (IL-9) was originally identified as T cell-derived T cell growth factor III/P40 that could support the long term growth of certain mouse T helper clones in the absence of antigen or antigen-presenting cells. Human IL-9 was independently cloned as a novel growth factor that is mitogenic for the human megakaryoblastic leukemic cell line, M07e. Human and mouse IL-9 share 56% identity at the amino acid level. Although mouse IL-9 is active on human cells, human IL-9 is not active on mouse cells.

Long Name

Interleukin 9

Alternate Names

IL9

Entrez Gene IDs

3578 (Human); 16198 (Mouse)

Gene Symbol

IL9

Additional IL-9 Products

Product Documents for Human IL-9 DuoSet ELISA

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-9 DuoSet ELISA

For research use only

Citations
Reviews