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Human Leptin R Quantikine ELISA Kit Best Seller

R&D Systems, part of Bio-Techne | Catalog # DOBR00

R&D Systems, part of Bio-Techne
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DOBR00

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.128 ng/mL

Assay Range

0.312-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Product Summary for Human Leptin R Quantikine ELISA Kit

The Quantikine Human Leptin sR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Leptin sR in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Leptin R/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Leptin sR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Leptin sR.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural and recombinant human Leptin sR

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 1.83 5.15 11.2 1.69 5.09 10.7
Standard Deviation 0.071 0.283 0.292 0.097 0.282 0.633
CV% 3.9 5.5 2.6 5.7 5.5 5.9

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 2.34 7.01 14.6 2.34 6.95 14.3
Standard Deviation 0.142 0.155 0.709 0.202 0.370 0.966
CV% 6.1 2.2 4.9 8.6 5.3 6.7

Recovery for Human Leptin R Quantikine ELISA Kit

The recovery of human Leptin sR spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 91-102
EDTA Plasma (n=4) 104 92-115
Heparin Plasma (n=4) 103 92-113
Serum (n=4) 106 95-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Leptin sR were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Leptin R ELISA Linearity

Scientific Data Images for Human Leptin R Quantikine ELISA Kit

Human Leptin R ELISA Cell Culture Supernate Standard Curve

Human Leptin R ELISA Cell Culture Supernate Standard Curve

Human Leptin R ELISA Serum/Plasma Standard Curve

Human Leptin R ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Leptin R

Leptin receptor (OBR), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OBR, including a long form (OBRL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OBRS) lacking signal-transducing capabilities, have been identified.

Long Name

Leptin Receptor

Alternate Names

B219, CD295, LEPR, LeptinR, OB R

Entrez Gene IDs

3953 (Human); 16847 (Mouse)

Gene Symbol

LEPR

Additional Leptin R Products

Product Documents for Human Leptin R Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Leptin R Quantikine ELISA Kit

For research use only

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.