Human Leptin R Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DOBR00
Key Product Details
Assay Length
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Summary for Human Leptin R Quantikine ELISA Kit
Product Specifications
Assay Type
Format
Measurement
Detection Method
Conjugate
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 1.83 | 5.15 | 11.2 | 1.69 | 5.09 | 10.7 |
Standard Deviation | 0.071 | 0.283 | 0.292 | 0.097 | 0.282 | 0.633 |
CV% | 3.9 | 5.5 | 2.6 | 5.7 | 5.5 | 5.9 |
EDTA Plasma, Heparin Plasma, Serum
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 2.34 | 7.01 | 14.6 | 2.34 | 6.95 | 14.3 |
Standard Deviation | 0.142 | 0.155 | 0.709 | 0.202 | 0.370 | 0.966 |
CV% | 6.1 | 2.2 | 4.9 | 8.6 | 5.3 | 6.7 |
Recovery for Human Leptin R Quantikine ELISA Kit
The recovery of human Leptin sR spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 96 | 91-102 |
EDTA Plasma (n=4) | 104 | 92-115 |
Heparin Plasma (n=4) | 103 | 92-113 |
Serum (n=4) | 106 | 95-114 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Leptin sR were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Leptin R Quantikine ELISA Kit
Human Leptin R ELISA Cell Culture Supernate Standard Curve
Human Leptin R ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Leptin R
Leptin receptor (OBR), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OBR, including a long form (OBRL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OBRS) lacking signal-transducing capabilities, have been identified.
Long Name
Alternate Names
Gene Symbol
Additional Leptin R Products
Product Documents for Human Leptin R Quantikine ELISA Kit
Product Specific Notices for Human Leptin R Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.