Human MBL Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DMBL00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.029 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human MBL Quantikine ELISA Kit
The Quantikine Human MBL immunoassay is a 4.5 hour solid phase ELISA designed to measure human MBL in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human MBL and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MBL showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of naturally occurring human MBL.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Oligomeric forms of natural and recombinant human MBL
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 0.91 | 2.05 | 4.06 | 1.02 | 2.06 | 4.14 |
| Standard Deviation | 0.03 | 0.07 | 0.22 | 0.08 | 0.14 | 0.29 |
| CV% | 3.3 | 3.4 | 5.4 | 7.8 | 6.8 | 7.0 |
Recovery for Human MBL Quantikine ELISA Kit
The recovery of MBL spiked to various levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 113 | 106-117 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of natural MBL were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human MBL Quantikine ELISA Kit
Human MBL ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MBL
Long Name
Mannose Binding Lectin
Alternate Names
COLEC1, Collectin-1, MBL2, MBL2D, MBP1
Entrez Gene IDs
4153 (Human)
Gene Symbol
MBL2
Additional MBL Products
Product Documents for Human MBL Quantikine ELISA Kit
Product Specific Notices for Human MBL Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.