Human MIA Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DMIA00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Saliva (50 µL)
Sensitivity
8.46 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Product Summary for Human MIA Quantikine ELISA Kit
The Quantikine Human MIA Immunoassay is a 4.5 hour solid-phase ELISA designed to measure MIA in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant Human MIA and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MIA. Results obtained using natural human MIA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIA.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human MIA
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 158 | 315 | 622 | 150 | 308 | 627 |
| Standard Deviation | 5.50 | 10.9 | 17.8 | 7.02 | 15.7 | 37.8 |
| CV% | 3.5 | 3.5 | 2.9 | 4.7 | 5.1 | 6.0 |
Recovery for Human MIA Quantikine ELISA Kit
The recovery of MIA spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 101-107 |
| EDTA Plasma (n=4) | 98 | 85-115 |
| Heparin Plasma (n=4) | 99 | 85-113 |
| Saliva (n=4) | 102 | 86-110 |
| Serum (n=4) | 101 | 91-118 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MIA were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Human MIA Quantikine ELISA Kit
Human MIA ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MIA
Long Name
Melanoma Inhibitory Activity
Alternate Names
CD-RAP
Gene Symbol
MIA
Additional MIA Products
Product Documents for Human MIA Quantikine ELISA Kit
Product Specific Notices for Human MIA Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.