Human/Mouse Total Bcl-xL DuoSet IC ELISA
R&D Systems, part of Bio-Techne | Catalog # DYC894-2
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
625-20000 pg/mL
Sample Type
Cell Lysates
Reactivity
Human, Mouse
Human/Mouse Total Bcl-xL DuoSet IC ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
Product Summary for Human/Mouse Total Bcl-xL DuoSet IC ELISA
This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total Bcl-xL in cell lysates. An immobilized capture antibody specific for Bcl-xL binds both phosphorylated and unphosphorylated Bcl-xL. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
Cell lysates (100 µL)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human/Mouse Total Bcl-xL DuoSet IC ELISA
Standard Curve
The Human/Mouse Total Bcl-xL DuoSet® IC ELISA is specific for Bcl-xL as shown by Western Blot analysis of the protein bound by the capture antibody supplied in the kit.
Lysates prepared from A431 human epithelial carcinoma cells were incubated in wells coated with Total Bcl-xL Capture Antibody. Unbound material was removed by washing and bound material was solubilized in SDS gel sample buffer. Captured proteins were electrophoresed, transferred to a PVDF membrane, and immunoblotted with Total Bcl-xL Detection Antibody. Only a single band corresponding to human Bcl-xL was detected.
Amounts of Bcl-xL, as measured by the Human/Mouse Total Bcl-xL DuoSet® IC ELISA, are consistent with the relative amounts of human and mouse Bcl-xL determined by qualitative Western Blot analysis.
A431 human epithelial carcinoma cells and NIH-3T3 mouse embryonic fibroblast cells were lysed as described in the Preparation of Samples section in the product insert (Catalog # DYC894). Human and mouse Bcl-xL were measured with this DuoSet® IC ELISA. The same lysates were immunoblotted (inset) with rat anti-mouse Bcl-xL. The DuoSet® IC ELISA results correlate with the relative amounts of Bcl-xL detected by Western Blot.Kit Contents for Human/Mouse Total Bcl-xL DuoSet IC ELISA
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
- Streptavidin-HRP
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Bcl-xL
Long Name
B Cell Lymphoma/Leukemia x Long Form
Alternate Names
BclxL
Gene Symbol
BCL2L1
Additional Bcl-xL Products
Product Documents for Human/Mouse Total Bcl-xL DuoSet IC ELISA
Product Specific Notices for Human/Mouse Total Bcl-xL DuoSet IC ELISA
For research use only
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